Thursday, November 28, 2019

Metamorphosis Essays (575 words) - Absurdist Fiction,

Metamorphosis Normal? In society today, there are many stereotypes and prejudices against people who do not fit into the norms of society. Society tends to regard these people as outsiders and therefore alienate them from many common social activities. One of the most prominent examples of people who suffer from this alienation are those with the HIV virus, more commonly known as AIDS. AIDS victims suffer through many of the same difficulties and discriminations as the main character in Franz Kafka's short novel The Metamorphosis. Throughout The Metamorphosis, Gregor's family serves to represent society, while Gregor himself serves as a direct representation of anyone who does not fit into the norms of society, in particular those people who suffer from AIDS. In the beginning of The Metamorphosis, the main character, Gregor, is transformed into a bug overnight and soon learns the difficulty of not being normal. He learns to live in a way that does not disturb society, or even his own family. Prior to becoming a bug, Gregor was a hard working salesman who was under appreciated by his family. After his transformation, Gregor is neglected by his family, with the exception of Grete, and the family soon disregards Gregor as one of their own, just as society disregards people who are not normal. Gregor's father can be compared to the abusive people in society. He is the dominant figure of the family and remains abusive throughout the story. The father's aggressiveness is revealed in the beginning of the story when he knocks gently, yet with his fist (217) on Gregor's door. Using his fist to knock on his son's door implies a sense of urgency and aggressiveness in his character. While there are many abusive people in society, at the same time the re are many people who are caring. Grete represents the caring people in society, but even the most caring people can become hateful. She takes on the responsibility of caring for Gregor and maintains that Gregor is still part of the family. However, as the story progresses she becomes irritated with Gregor and views him as a burden to the family. Although having AIDS is not as obvious as being transformed into a bug, AIDS can cause the same devastating effects once it is revealed. The most common fear that people in society have of AIDS is interacting with someone who has the virus. Gregor experienced this same fear of interaction. This fear is most noticeable when Grete is playing the violin for the house guests. Gregor walks in to the living room and, after seeing Gregor, the house guests become upset because of the disgusting conditions (240) of the family. Another similarity between Gregor's illness and AIDS is that there is no cure. Both Gregor and AIDS victims have to live knowing that they cannot change the way they are, which makes them indifferent towards their appearance. By revealing himself to the house guests, Gregor demonstrates this indifference and offends the guests. Both Gregor and AIDS victims learn to live with the many limitations set forth by society. However, these limitations can be overwhelming and extremely exhausting at times. Through his family's reaction, Gregor experiences a common theme in society today; if you are not considered normal by society's standards, then you cannot live a normal life. In the end Gregor realizes that he cannot relieve the mental and physical strains of being a bug and decides that death English Essays

Monday, November 25, 2019

Bermuda Essays - Bermuda Triangle, Flight 19, Bermuda, Free Essays

Bermuda Essays - Bermuda Triangle, Flight 19, Bermuda, Free Essays Bermuda *A legendary triangle of Ocean lies between 3 countries upon the Atlantic *ocean. The Cities are Bermuda, Puerto Rico and Fort Lauderdale. Ships, *people and aeroplanes have been reported mysteriously disappearing off the *face of the earth whilst travelling inside this triangle. It soon acquired *the name Devils Triangle owing to peoples superstitions that the devil *was at play on this stretch of ocean and gobbling up weary and lost *travellers with great delight, but what actually was at play inside this *triangle of rough water, is it really the devil?, or perhaps aliens are *using this spot as their home base on earth. Maybe it really does contain a *mystical vortex that sucks people down into a third dimension. * *The myth of the mysterious triangle was first begun in an Associated Press *dispatch of September 16, 1950. Reporter E.V. W. Jones wrote of mysterious *disappearances of ships and planes between the Florida coast and Bermuda. *Two years after this article appeared Fate magazine ran an article by *George X. Sand about a series of strange marine disappearances, each *leaving no trace whatever, that have taken place in the past few years in *a watery triangle bounded roughly by Florida, Bermuda and Puerto Rico. * *It was not long before ideas and suggestions started forming about this *piece of ocean. M.K. Jessup wrote about the disappearances and gave ideas *about alien intelligences being behind them in the book The Case for the *UFO. The view was also echoed by Donald E. Kyhoe who is noted for his The *Flying Saucer Conspiracy of 1955. Frank Edwards (Stranger Than Science) *agreed with the theory of aliens having a local hangout in the triangle as *well. Finally a man by the name of Vincent H. Gaddis came up with the *phrase Bermuda Triangle. * *Vincent Gaddis wrote an article in February 1964's edition of Argosy and *incorporated the story later in his book Invisible Horizons titled The *Deadly Bermuda Triangle. And there was the birth of the now world famous *myth of the Bermuda Triangle. * *Throughout the years it has featured in many many articles, books, *television series and movies and always portrayed as a very real and *mysterious thing, but anyone out there with any sense surely will ask *themselves how in this day and age could boats, planes and other *travellers just go mysteriously missing in a certain piece of water? *wouldn't an aeroplane full of international travellers be afraid to travel *over this part of the ocean....Well let me tell you I've always asked the *same questions myself and I personally have come to the conclusion that the *entire thing is nothing more than a myth hyped up over the years by wrong *facts and silly overexaggerated stories told down through the generations *of people willing to listen to any kind of mysterious story with a unknown *edge to it. * * * *One of the most famous stories to ever surround the Bermuda Triangle is the *mysterious disappearance of the Naval Air Flight 19. * *So what happened? * *On December 5th, 1945, five Avenger torpedo bombers left the Naval Air *Station at Fort Lauderdale. They never returned home. * *The Avenger bombers contained 14 men, 13 of those were trainees in the last *stages of their training along with Lt. Charles Taylor. The five pilots had *been recently transferred from the Miami Naval Air Station. Lt. Taylor knew *the Florida Keys well but had no knowledge of flying over the Bahamas which *was the direction Flight 19 was headed in. * *Their mission on that day was for practice bombing at hens and Chicken *Shoals fifty-six miles away. Once that was accomplished, the Avengers were *to continue on eastward for another sixty-seven miles, then head north *seventy-three miles. Following that they would turn southwest and head for *home. In other words they were flying a triangular flight path through what *would be called the Bermuda Triangle. * *At 3.50pm that afternoon a pilot and his flight instructor, Lt. Robert Cox *were about to land at Fort Lauderdale. They overheard a radio transmission *addressed to someone named Powers. Powers replied, I don't know where we *are. We must have got lost after that last turn. * *A little later on Lt. Cox managed to establish radio contact with another *of the Pilots

Thursday, November 21, 2019

Legal Aspects of Nursing Final Proyect Essay Example | Topics and Well Written Essays - 1500 words

Legal Aspects of Nursing Final Proyect - Essay Example A registered nurse is obligated to use her capabilities and decisions to make resolutions on whether to take on responsibilities or whether to assign the responsibility to another person (Florida Department of Health & Florida Board of Nursing, 2007). A certified nurse should offer care and help that are associated to personal care, maintaining mobility, hydration and nutrition, elimination, use of assistive devices, sustaining the environment and resident safety, data gathering, and post mortem care. In addition, a certified nurse should offer care related to identification of and reporting of abnormal resident findings, symptoms and signs, roles associated with end of life care, resident socialization, leisure activities, and legalization methods and realism course. A registered nurse should perform duties associated with basic first aid, emergency care, and CPR abilities, duties associated with documentation of certified nursing services on daily basis (Florida Department of Healt h & Florida Board of Nursing, 2007). Duties associated with personal care include: dressing, bathing, grooming, shampooing and care of hair, shaving, caring for the skin, feet, and nails, offering and helping with oral hygiene and denture care, offering pericare, sustaining a clean environment, and making a patient’s bed and handling the patient’s linen. ... In the use of assistive devices, a registered nurse has the responsibility of taking care of eyeglasses and contact lenses, applying braces, antiembolus stockings, wheel chairs and crutches, and using comfort devices such as wedges, pillows, boots and wedges (Florida Department of Health & Florida Board of Nursing, 20007). The Florida nursing practice act has the duty to practice his or her profession stanchly, desist from deliberately taking harmful drugs, and desist from overseeing any injurious drug to patients, sustain and elevate standards of the nursing profession, and maintain any confidential information of patients during the practice. In addition, a registered nurse should assist physicians in their practice, follow instituted nursing procedures, follow the instructions of their supervisors, and advocate for his or her patient (Florida Department of Health & Florida Board of Nursing, 2007). Before being licensed as a nurse, an individual should fill an application form and submit a fee that does not exceed $150 set by the board and an examination fee that does not exceed $75. In addition, the individual should pay applicant cost to the board for the purchase of an examination from the National Council of State Boards of Nursing. Before licensure, an individual should also provide adequate information, which must be presented by the department for a nationwide criminal records correspondence check through the department of law enforcement. Before being licensed, an individual should also be physically and mentally sound. In addition, one should be a holder of a high school diploma, and has accomplished the conditions for

Wednesday, November 20, 2019

A Basic Project Management Framework and the Proper Staff Literature review

A Basic Project Management Framework and the Proper Staff - Literature review Example The measures that had been put in place were not working, in July emergency measures were put in place.   This included free two-year extensions to passports as well as many other drastic measures.   One such measure was to add 100 staff to places issuing passports, and there was a phone line set up to deal with inquiries over the phone. In addition, more measures were put in place in order to improve the lines at passport offices.   Even though the Agency set up a goal of reaching the deadlines for processing passports by peoples’ dates of departure, there were still about a half of a grand missed dates of departure.   This is not to mention all of the hand-wringing going on by customers who were anxiously anticipating their passports being processed. Another aspect of this whole fiasco was that, throughout the ‘90s, some of the concerns of management were to lessen costs as well as make the organization more effective, at the same time keeping good quality cust omer relations.   Management thought that perhaps the Agency had done well for itself, improving in both of these senses of the word and that it boded well with other agencies comparable to itself—costs to the consumer being competitive with other places around the country. Regardless, both management and the Agency realized that they had not taken into consideration, along with the fact that there was increased demand for passports, that the Agency was working out of deference to how many passports were coming in at one time rather than taking into account the fact that the Agency was dealing with a higher demand.  Ã‚  

Monday, November 18, 2019

The Constitution and the U.S. Government branches Assignment

The Constitution and the U.S. Government branches - Assignment Example To gain efficiency in subsequence, the US Constitution has significantly divided the federal government into three branches i.e. Legislative Branch, Executive Branch and Judiciary Branch (Trethan, 2013). Article 1 of the US constitution describes its Legislative Branch, which includes two main bodies – namely ‘House of Representatives’ and ‘Senate’, collectively known as Congress. These bodies are vested with the authority to frame laws for the nation. Article 2, correspondingly describes the Executive Branch, which is governed by the US President. The President of the nation executes the federal laws as well as makes necessary recommendation regarding new laws (Minnesota House of Representatives Public Information Services, 2014). Additionally, in accordance to this branch, the President directs the national defense as well as formulates foreign policies and endures the various ceremonial duties. The third constitutional branch, as mentioned under the US Constitution is the Judicial Branch. Article 3 of the constitution herewith covers the Judicial Branch, which is headed by the US Supreme Court. In this regard, the key power of this branch is to un derstand and evaluate the entire Constitution, appraise laws and make decisions relevant to various cases that are engaged with the rights of the states (Mount,

Friday, November 15, 2019

GFP Transformation Into E Coli Biology Essay

GFP Transformation Into E Coli Biology Essay Genetic transformation  is the technique of introducing a recombinant DNA into a living cell. In this experiment, we introduced pGLO plasmid into E. Coli bacteria through the heat-shock method. CaCl2 solution was used to make the E. coli cells competent. Intruduction Genetic transformation is the technique involving introduction and expression of foreign(exogenous) DNA in a living host cells. Scientists cut out interested gene from human, plants or animals DNA, insert it into a vector to produce a recombinant DNA and introduce the recombinant DNA into host cells which express the exogenous genes and produce interested proteins under appropriate condition. Demonstrated by Frederick Griffith in 1928, transformation has been applied in various areas of biotechonology. For example, genes coding for human insulin can be genetically transformed into bacterial cells. In this way, genetic transformation allows the production of protein products on a large scale. The introduction of a foreign DNA into a host cell requires the use of a vector. Vectors are small DNA molecules that can be used to combined with foreign genes and transfer them into the host cells. In a laboratory research, plasmid is one of the most commonly used vectors to transform foreign DNA. The recombinant plasmid used in this experiment is pGLO plasmids. pGLO plasmids contain  two genes that are useful: the gene coding for beta-lactamase and the gene coding for the green  ¬Ã¢â‚¬Å¡uorescent protein(GFP). The expression of beta-lactamase gene provides resistance to the antibiotic ampicillin. GFP is extracted from Aequorea victoria (bioluminescent jelly fish). It is a polypeptide consisting of 238 amino acid(Cubitt et al., 1995). For its non-invasive characteristic and capacity of resisting interferences, GFP has been widely used as a visual marker for gene expression for more than 20 years(Gilbert et al., 2000). Even under normal light, GFP presents yellow-green color that can be easily observed. The objects of this experiment were to perform a transformation of E. coli with a plasmid containing the GFP DNA gene and force the GPF to be expressed in a particular environment. In this experiment, we induced E. coli to take in pGLO into their cytoplasm and grew the E. coli cells in different plates. CaCl2 solution is utilized as transformation solution to increase cell membrane  permeability, thus plasmid vectors are able to enter the cell. The ampicillin-resistance gene in the plasmid was utilized as the selectable marker, which means E. coli cells transformed with plasmid are able to grow in an environment with ampicilin. Because the GPF expression is driven by the arabinose-stimulated PBAD promoter, it is expected that the E. coli cells grown in the plate with sugar arabinose would express GPF DNA and present a yellow-green color in the UV light. Materials and Methods Plasmid Transformation Plasmid could be easily taken in by E. coli cells if the cells had been treated with calcium salt. First of all, two micro test tubes were labeled with either +pGLO or -pGLO. Each tube was added with 250ÃŽ ¼L of CaCl2 solution and then placed on ice. A single colony of E. coli bacteria was added into each tube. The cells were dissolved into the solution by gently spinning the tubes. Then both tubes were placed on ice again. After the entire colony was dispersed, the tubes were examined with UV light to make sure that there were no visible green clumps of cells in the solution. Then 10ÃŽ ¼L of pGLO plasmid was added into the +pGLO tube and gently mixed, while no plasmid was added into -pGLO tube. Both tubes were placed on ice for 10 minutes. Following the 10-min incubation, the tubes were heat shocked. Both tubes were transferred into 42 °C water bath for exactly 50 seconds, and then immediately placed back on ice for 10 minutes. The heat shock process must be rapid. After heat shock, 250ÃŽ ¼L of Luria broth (LB) were added to each tube and the tubes were placed at room temperature for 10 minutes. Selection of GFP Transformation To grow and select the cells with GFP DNA, four agar plates were obtained: 1 LB, 2 LB/AMP and 1 LB/AMP/arabinose. Among them, 1 LB/AMP plate and 1 LB/AMP/arabinose plate were labeled with +pGLO, while 1 LB plate and 1 LB/AMP plate were labeled with -pGLO. 100ÃŽ ¼L of cells from +pGLO tube was added to each of the plates labeled with +pGLO, while 100ÃŽ ¼L of cells from -pGLO was added to each of the plates labeled with -pGLO. New sterile loops was used for each plate. The whole process was conducted near fire. Then four plates were stached upside down at 37 °C for 24 hours. A photo of the plates were taken in the UV light the next day. Results Figure 1 on the next page shows a photo of four plates. Table 1on the next page illustrates the observation results of each plate. From the photo, we can observe the growth condition of colonies on each of the four plate. Plate 1: the untransformed E. coli colonies grew normally and form a lawn on the plate; Plate 2: no colonies grew; Plate 3: transformed E. coli colonies grew and fluorescenced yellow-green in UV light; Plate 4: E. coli colonies grew and presented white color in UV light. Fig Photo was taken after the plates had been incubate at 37 °C for 24 hours. Table Observation of the colonies in each of the four plate Plates Sample Colonies 1 LB E. coli -pGLO plasmid lawn 2 LB/amp E. coli -pGLO plasmid no colonies 3 LB/amp/arabinose E. coli +pGLO plasmid yellow-green colonies 4 LB/amp E. coli +pGLO plasmid white colonies Discussion In the plate containing LB and E.coli -pGLO (Plate 1), bacterial cells formed a lawn, because there was no antibiotic in the medium. The cells grew normally as in natural condition. Plate 1 is a negative control which excludes possible contaminants. In the plate containing LB/amp and E.coli -pGLO(Plate 2), no colonies grew, because the ampicillin  in the medium killed the cells by inhibiting the cell wall from producing. In the plate containing LB/amp/ara and E.coli +pGLO(Plate 3), colonies were grew, because these bacterial cells contains plasmid carrying ampR gene which perform a resistance against ampicillin. Under UV light, the colonies emit green-yellow fluorescence, because the GFP gene on plasmid was expressed in an environment with arabinose. In the plate containing LB/amp and E.coli +pGLO(Plate 4), white colonies were grew because of the presence of ampR gene and absence of arabinose. The presence of ampicillin in the medium is to identify if the E.coli cells have taken in plasmid and thus acquired ampicillin-resistance gene on the plasmid. The results of Plate 4 compared with Plate 2 indicate that E.coli cells have taken in plasmid successfully. The presence of arabinose is to identify if the E.coli cells containing recombinant plasmid successfully inserted with GFP gene. The results of Plate 3 compared with Plate 4 indicate that the plasmid was successfully recombined, thus the cells express GFP under the induction of arabinose. Conclusion In this experiment, GFP transformation in E. coli was perform in order to examine how the recombinant plasmid can be introduced into bacterial cells, incorporated into bacterial genome and express recombinant protein. The experiment shows the expected results, which successfully support the hypothesis.

Wednesday, November 13, 2019

Palestine Dispute Essay -- essays research papers fc

  Ã‚  Ã‚  Ã‚  Ã‚  After the defeat of the Ottoman Empire in World War I, the control of Palestine shifted from Muslim to Western powers. Britain promised Jews and Zionists the land in Palestine in the form of the Balfour Declaration. However, at the same time, there were already Palestinians living in that land. Even today, there is no correct answer as to who should have rightfully acquired the land; however, the Palestinian Arabs should have cooperated when the Jews immigrated to the Palestine. There are plenty of facts which support both sides. The solution can only be determined through one’s opinion.   Ã‚  Ã‚  Ã‚  Ã‚  According to Biblical sources, Arabs and Israelites first met in Palestine about 1500 to 500 B.C. This time period recognized the beginning of the tradition of the two peoples’ common patriarchs and the cousinly ties between the offspring of Isaac and the children of Ishmael, the sons of Abraham.(Cohen 1)   Ã‚  Ã‚  Ã‚  Ã‚  Before Islam surfaced, the Jews and the Arabs met in the Arabian peninsula. There was actually a very large Jewish population in the Arabian peninsula before the Prophet Muhammed was known. After the Jews were kicked out of Jerusalem by the Romans following the Second Revolt, a large number of Jews went to the northern area of the Arabian peninsula. The settlements of Jews in the Valley of the Villages were important areas for the wandering and nomadic Arabic tribes. The Arabs actually settled near them under their protection, understanding their traditions, customs, and views of religious and social matters. Before Muhammed, some Arabs even accepted the Jewish doctrine of the â€Å"chosen people† and other basic elements of Judaism. (Cohen 2)   Ã‚  Ã‚  Ã‚  Ã‚  However after some time, things started to change. The prophet Muhammed began presenting Islam as a new religion. He even tried converting some Jews into Islam. But when his hopes of winning them over failed, he turned against the Jews. In 626, he attacked the Jewish tribes of Medina and completely destroyed them. This marked the beginning of conflict between the Jews and the Arabs. (Cohen 3) The relationship between the Jews and the Arabs always remained tense. The connection between the two peoples is tense even today. Even with the same enmity, there has been a great deal of change from the beginning of time. An impor... ...o solution to the Arab-Israeli problem. The wars are merely eruptions in the historical encounter that is nothing less than a prolonged war which is doomed to continue until it is resolved in a catastrophic holocaust.   Ã‚  Ã‚  Ã‚  Ã‚  However, others actually have a standpoint. Some believe that the Jews really deserved the land in Palestine because it was theirs to begin with. They were forced out of Jerusalem by the Romans. On the other hand, others say that the land is rightfully the Arab’s land. There were Palestinian Arabs who were living there before the Jews were rewarded the land by the British. Why should they give up their land? There is no solution. However, the two peoples could have lived peacefully. The two groups could have lived together if the Arabs had cooperated. If the Arabs already living in the land had not initiated the attacks on the Jews, the two peoples could have eventually learned to live together in one nation. Works Cited Barker, A. J. Arab-Israeli Wars. New York: Hippocrene Books, 1980. Cohen, Aharon. Israel and the Arab World. New York: Funk & Wagnalls, 1970. Levin, Meyer. The Story of Israel. New York: G.P. Putnam's Sons, 1966.